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Literature summary for 3.2.1.2 extracted from
- Production of recombinant beta-amylase of Bacillus aryabhattai (2019), Prep. Biochem. Biotechnol., 49, 88-94 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant enzyme expression in Bacillus choshinensis using the constitutive P2 promoter, derived from a cell-wall protein of the host bacterium, as the expression promoter for the beta-amylase | Priestia aryabhattai |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | analysis of the effects of carbon source, nitrogen source, and metal ions on cell growth and Bacillus aryabhattai beta-amylase production in recombinant Brevibacillus choshinensis. The optimal medium contains 7.5 g/l glucose, pig bone peptone (40.0 g/l), Mg2+ (0.05 mol/l), and trace metal elements, the beta-amylase yield reaches 925.4U /ml, which is 7.2fold higher than that obtained in the initial medium. A modified feeding strategy is proposed and applied in a 3-l fermentor fed with glucose achieving a dry cell weight of 15.4 g/l. Through this cultivation approached 30°C with 0 g/l initial glucose concentration, the maximum beta-amylase activity reaches 5371.8 U/ml which is 41.7fold higher than that obtained with the initial medium | Priestia aryabhattai |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Priestia aryabhattai |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Priestia aryabhattai | |
| Mg2+ | activates, maximum enzyme activity of 925.4 U/ml is achieved in the presence of 50 mM Mg2+ | Priestia aryabhattai | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia aryabhattai | - |
- |
- |
| Priestia aryabhattai CCTCC M2017320 | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | analysis of the effects of carbon source, nitrogen source, and metal ions on cell growth and Bacillus aryabhattai beta-amylase production in recombinant Brevibacillus choshinensis. The optimal medium contains 7.5 g/l glucose, pig bone peptone (40.0 g/l), Mg2+ (0.05 mol/l), and trace metal elements, the beta-amylase yield reaches 925.4U /ml, which is 7.2fold higher than that obtained in the initial medium. A modified feeding strategy is proposed and applied in a 3-l fermentor fed with glucose achieving a dry cell weight of 15.4 g/l. Through this cultivation approached 30°C with 0 g/l initial glucose concentration, the maximum beta-amylase activity reaches 5371.8 U/ml which is 41.7fold higher than that obtained with the initial medium. Cell growth and protein synthesis reach a balance at 30°C | Priestia aryabhattai | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-1,4-glucan maltohydrolase | - |
Priestia aryabhattai |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Priestia aryabhattai |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Priestia aryabhattai |
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Release 2023.1 (January 2023)
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